Okay, I'll go ahead and get the Morris Day line from Purple Rain out of the way: "How's the family?"

I also cracked up during the scene in Predator 2, when the Predator bursts through the old ladies apartment building leaving destruction in his path. Danny Glover, in pursuit, shows the lady his badge and assures her, "It's okay, I'm a cop". She simply replies, "I don't think he gives a shit!"

What are some others?

Aliens - "Eat this."

And Die Hard Trilogy..there are some of the best one liners I've ever heard! Such a shame I don't remember them

This one is classic:

"I'll have what she's having."

ernestsewell said:

When Harry Met Sally

"That's right, I did it. I killed Yvette. I hated her... SO MUCH... it was flaming, flames, FLAMES... on the side of my face... heaving breaths, heaving... "

Don't knock masturbation. It's sex with someone I love.

Most of Donkeys lines on Shrek 1

Viggy,viggy,viggy...you have been a bad monkey

From the movie Friday

Smokey: You got to be a stupid motherfucker to get fired on your day off.

and

Smokey: Puff puff, give. Puff puff, give. You fuckin' up the rotation.

TheVoid said:

Madeline Kahn was so damn awesome!!!

Rip Torns "You all are about as useful as a cock-flavored lollipop" from Dodgeball had me pissing myself when I first heard it.

Kiss Kiss Bang Bang has plenty....

Harry: "Don't worry, I saw Lord of the Rings. I'm not going to end this 17 times."

Harmony: Well, for starters, she's been fucked more times than she's had a hot meal.
Harry: Yeah, I heard about that. It was neck-and-neck and then she skipped lunch.

muirdo said:

For some reason, I don't remember that line. That's hilarious though.

xpertluva said:

The American version said poop instead of cock apparently.

I know its not a line but one of the funniest moments in film I ever saw was from The Big Lebowski when they are scattering Donnies ashes

the best version i can find on youtube


[Edited 4/2/10 12:15pm]

The black marine in Aliens :"Guess she didn't like the cornbread neither"

or.....



Peter Sellers was fantastic in this

Dr. Strangelove is one of my all-time favorite movies and the call to the Russian Premiere is my favorite part.

xlr8r said:

Frost

Childs Play - The Scene in the elevator where the lady looks at Chucky and say "What an ugly doll" when they leave Chucky says "Fuck You!!!!"

Blazing Sadles - "Where all the white women at!!"

THE HANGOVER

Alan Garner: I'm not supposed to be within two hundred feet of a school... or a Chuck E. Cheese.

Alan Garner: Tigers love pepper... they hate cinnamon.

Alan Garner: Oh, you know what? Next week's no good for me... The Jonas Brothers are in town. But any week after that, it's totally fine.

Mr. Chow: Its funny because he's fat!

Phil Wenneck: Jesus, he's like a gremlin. Comes with instructions and shit.

Stu Price: Ew! Alan, did you just eat sofa pizza?

Officer Franklin: Not you, fat Jesus.

Stu Price: Here's something I would like to remind you two of: our best friend Doug is probably face down in a ditch right now with a meth head butt-fucking his corpse!

Alan Garner: It's not illegal. It's frowned upon, like masturbating on an airplane.

Stu Price: We're in a stolen cop car with what is sure to be a missing child in the back. What part of this is cool?

Mr. Chow: Oh yeah? Why dont you suck on these little Chinese nuts?


SUPERBAD

Evan: Wait... you changed your name to... McLovin?

Fogell: Gangstaaaaasss... what's up guys?

Evan: Fogell, shut the fuck up. And take off that vest. You look like Aladdin.

Officer Slater: McLovin? Were you violating that young girl? Were you violating her with your penis?

Seth: You know when you hear girls say 'Ah man, I was so shit-faced last night, I shouldn't have fucked that guy?' We could be that mistake!

Officer Michaels: I'm assuming you all have guns and crack!

Seth: I'll be like the Iron Chef of pounding vag.

Officer Slater: I'm sorry that I blocked your cock...

Officer Michaels: Fogell? Fuck that, we're calling you McLovin!

Officer Michaels: Prepare to be fucked by the long dick of the law!

Acrylic said:

The fat guy is hilarious,,,I damn near pissed on myself when he said What are you? An Irish R&B singer?

xpertluva said:

My two favorites I can think of is Sigourney Weaver in Aliens 2: "GET AWAY FROM HER YOU BITCH!" LOL and one of the most ironic and funny scenes in cinematic history where Robert Duvall (along with his thrill at surfing and bombing to the tune of Wagner's "Ride of the Valkyries") states: "I love the smell of napalm in the morning..."

"AK-47! When you absolutely, positively have to kill every !@#$%^ in the room, accept no substitute!"

-Sam Jackson as Ordell, Jackie Brown

The Big Lebowski


Nobody fucks with the Jesus

and

Say what you will about the tenet's of National Socialism, Dude -At least it's an Etho's

"It ain't cool to be no jive turkey so close to Thanksgiving" - trading places (?)

Enter the Dragon - "Man, you straight out of a comic book." The funniest part about that line is I'm not sure if it was supposed to be funny or not.

Chris Tucker from Dead Presidents (paraphrased):

"My great-great granddaddy was a pimp...he used to make his hoes pick his cotton for him!"

...and from Friday:

"Girl said she looked like Janet Jackson...got out the car lookin like Freddie Jackson."

Samuel L from Pulp Fiction:

"Sewer rat may taste like pumpkin pie but I'll never know cause I ain't gonna eat the filthy mothafucka."

"I'm the fuckin foot master!"

"Look at the big brain on Bret...you a smart mothafucka...that's right!"

Brad Pitt from "Kalifornia":

"In California first months rent is free, state law. So I figure we'll just move around for awhile."